1.
Indian J Exp Biol
;
1996 Nov; 34(11): 1169-71
Article
in English
| IMSEAR
| ID: sea-59303
ABSTRACT
A pair of oligomers of 20 and 23 bp were designed for amplifying a 381 bp sequence from glycoprotein IV gene of bovine herpesvirus 1. The primer pairs were used for amplifying genomic DNA of BHV-1 directly from cell culture fluids under different experimental conditions such as, untreated cell culture fluid, thermal denaturation and proteinase K treatment in presence of detergent. The results reveal that direct thermal denaturation of cell culture fluid is sufficient to detect the virus by polymerase chain reaction.